Isotype Controls, FMOs, Both, or None?

Once a compensation matrix is calculated to correct for fluorescence spillover, more controls must be run to ensure an analyte’s signal is real. These controls include fluorescence minus one (FMO) and isotype controls. FMO controls contain every antibody in your panel except for one. FMOs are useful as a gating control to identify the gating boundary for the one antibody that is missing. FMO controls are mainly used to identify background staining due to fluorescence spillover. Isotype controls are useful for identifying nonspecific staining. The isotype control should have the same heavy chain isotype, the same light chain, the same color, and be derived from the same species as the antigen-specific antibody. For example, Tonbo APC Rat IgG1 Isotype Control (cat. no. 20-4301) should be paired with APC Rat Anti-Mouse IFN gamma (cat. no. 20-7311). Isotype controls should be used at the same concentration as the antibody for which it is a control. Ideally, the isotype control antibody should be purchased from the same company as the test antibody, especially for tandem dye conjugates as manufacturing processes may vary from vendor to vendor. […]

By | February 21st, 2018|News|0 Comments

The Holidays are Right Around the Corner

  Offer applies to any of these products: Flow Cytometry Antibodies PCR Reagents Recombinant Proteins Use Promo-code: "TONBO-ACTIVATE" Also, get a $25 Espresso Pod Gift Card Use Promo-code: "MORE-ESPRESSO" (with a $400 minimum purchase) Share Tweet Forward Nespresso® is a registered trade mark of Societe Des Produits Nestle SA. Promotion Restrictions: Must purchase $1,000 or [...]

By | November 18th, 2016|News|0 Comments

Introducing Ghost Violet 540!

  Ghost Violet 540 Just like the other Tonbo Ghost Dyes™, Ghost Violet 540 allows for clear live vs. dead cell discrimination for more precise flow cytometry analysis. Excitation: 405 nm (Violet Laser) Emission: 540 nm Order today and get Ghost Violet in your lab! Order Here

By | November 3rd, 2016|News|0 Comments

Introducing Tonbo’s advanced Endpoint and Real-Time PCR reagents

Tonbo’s advanced Endpoint and Real-Time reagents engineered to maximize yield, specificity, sensitivity & speed.  Providing the molecular immunologist with convenient tools for even the most challenging PCR reactions - try them today!

By | August 12th, 2015|News|0 Comments

Separation Anxiety? Save on LSM

Lymphocyte Separation Medium (LSM) is a sterile filtered iso-osmotic solution designed for isolation of lymphocytes from diluted whole blood.

By | August 3rd, 2015|News|0 Comments

Cy –> Cyanine

Over the next 1-2 months Tonbo products that include ‘Cy’ as part of their name will begin to change to ‘Cyanine’. This includes APC-Cy7, PE-Cy5, PE-Cy7 and PerCP-Cy5.5 formats, corresponding to Tonbo catalog number prefixes 25, 55, 60 and 65. Changes will first appear on the website, followed by updated Technical Data Sheets. Product labels [...]

By | August 1st, 2015|News|0 Comments

Ready! Set! Activate!…or Block, Neutralize, Deplete!

Look to Tonbo for a wide selection of mouse and human antibodies in our no azide, low endotoxin and carrier-free In Vivo Ready™ (IVR) format. Endotoxin levels less than 0.01 EU/µg of protein means they are ideal for sensitive in vivo or in vitro assays like neutralization, blocking or activation. We are also happy to offer any of our existing clones in IVR [...]

By | July 13th, 2015|News|0 Comments

Validated reagents. Trusted by your peers.

Check out what researchers around the world are doing with reagents from Tonbo. Our flow and In Vivo Ready antibodies, buffers, and RPx Pro™ recombinant proteins are being cited in top immunology journals. We provide quality, validated reagents to support your immunology workflow - now you can read about them in a growing selection of [...]

By | June 30th, 2015|News|0 Comments

Providing an integrated portfolio for your immunology workflow

Tonbo Biosciences has the tools you need for immunology research. Our antibodies, reagents and support products provide unparalleled value at every step in your workflow. Need bulk or custom services? We do that too.

By | May 27th, 2015|News|0 Comments

New! PCR product line!

Our PCR products feature: Advanced reaction buffers rigorously developed to provide optimal success with a variety of applications with little to no protocol optimization --  easy-to-use, robust performance Enzymes engineered to increase yield, sensitivity, specificity, and speed dNTPs and MgCl2 included in the pre-optimized reaction buffer for convenience and value Most Endpoint products available as [...]

By | May 21st, 2015|News|0 Comments