Cell Stimulation Cocktail (500X) Cat. No. TNB-4975-UL100
Note: Cell Stimulation Cocktail is supplied as a 500X solution in Ethanol. Product should be stored at 20°C, protected from light and tightly sealed to prevent evaporation.
Other Materials Required
- RPMI complete medium (RPMI with 10% FBS, 1% pen/strep, 50 µM 2ME)
For activation of mouse splenocytes:
- Harvest spleen and prepare single cell suspension in RPMI complete medium at approximately 5-10 x 10^6 cells/mL.
- Add 1X (2 µL/mL) Cell Stimulation Cocktail (500X) directly to the cell suspension.
- Culture overnight (6-18 hours) at 37°C, 5% CO2 . We typically leave this culture overnight which will generate a nice population of IL2 and IFNgamma expressing cells.