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Tonbo Fluorochromes

Posted by Michaela Frister on Sep 21, 2014 12:17:23 PM

We are often asked for more details about our violetFluor™ 450 and redFluor™ 710 fluorochromes.

Below you’ll find information on excitation and emission, along with other common dyes ours can be substituted in for.

violetFluor™ 450 is excited by the violet (405 nm) laser and has a peak emission of ~450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™  450 can be used as an alternative for Pacific Blue®, BD Horizon™  V450 or eFluor® 450.

redFluor™ 710 is excited by the red (633-647 nm) laser and has a peak emission of ~710 nm. The recommended band pass filter for this dye is 710/50. redFluor™ 710 can be used as an alternative for Alexa Fluor® 700. Before use, we recommend confirming that your cytometer is configured to detect this fluorochrome. Note that while BV711 has a similar emission max, it is excited by the violet (405 nm) laser, so redFluor™ 710 would not be considered an alternative for BV711.

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Topics: emission, excitation, fluorochromes, News, redfluor, violetfluor

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